β-Hydroxybutyrate and Acetoacetate Levels

Measurement of blood acetoacetate and β-hydroxybutyrate in an automatic analyser

beta-hydroxybutyrate and acetoacetate as well as lactate and pyruvate are intermediary metabolites normally present in blood. The beta-hydroxybutyrate/acetoacetate ratio is an expression of the mitochondrial oxido-reduction state. This ketone body ratio can provide a clue to diagnosis and metabolic status in congenital errors of the electron transport chain and pyruvate metabolism. The standard...

Concentrations of Acetoacetate and D-(--)-3-hydroxybutyrate in Rat Liver and Blood.

Hydroxybutyrate, Acetoacetate, and Glucose for Respiration and Lipid Synthesis in the 1%Day-old Rat*

A comparison has been made in vivo between IS+)-3hydroxy[3-Wlbutyrate, o(-)-3-hydroxy[3-“‘Clbutyrate, [3Wlacetoacetate, and n-[Z-Wlglucose for sterol and fatty acid synthesis and respiration in the B-day-old suckling rat. (a) Sterols and fatty acids in spinal cord, brain, and skin were preferentially labeled by these metabolites over sterols and fatty acids in the liver and kidneys. (b) More la...

Multipoint kinetic method for simultaneously measuring the combined concentrations of acetoacetate-beta-hydroxybutyrate and lactate-pyruvate.

This is a multipoint kinetic method for simultaneously determining acetoacetate (AcAc) plus beta-hydroxybutyrate and lactate plus pyruvate in a single cuvette of the Multistat III centrifugal analyzer. In the first step, AcAc and pyruvate are completely reduced, using 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) and lactate dehydrogenase (EC 1.1.1.27) in the presence of excess NADH at pH 7.5, ...

β hydroxybutyrate levels in serum and cerebrospinal fluid under ketone body metabolism in rats

A high-fat, low-carbohydrate diet (KD) or calorie restriction in the form of every-other-day fasting (EODF) results in ketone body metabolism with an increasing β-hydroxybutyrate (βOHB) level. Previous studies have supported that a KD and EODF have a neuroprotective effect. However, the βOHB levels in the cerebrospinal fluid (CSF) resulting from a KD and EODF remain unknown. The aim of this stu...